Laboratory Evolution of High-Redox Potential Laccases

Directed Evolution of Fungal Laccases

Fungal laccases are generalists biocatalysts with potential applications that range from bioremediation to novel green processes. Fuelled by molecular oxygen, these enzymes can act on dozens of molecules of different chemical nature, and with the help of redox mediators, their spectrum of oxidizable substrates is further pushed towards xenobiotic compounds (pesticides, industrial dyes, PAHs), b...

How to Change the Redox Potential of Guanine?

Due to some important applications of guanine electrode in scientific and technology research such as electro-chemical DNA based biosensors, and a problem of high redox potential of guanine (0.81 V), our research study concentrates on reducing guanine redox potential by substituting Cu, Ag, Au, CH3, C2H5 and Cl on sites of 1, 2 and 9 of guanine. A 5.0% reduction...

Collectorless flotation of Chalcocite by controlling redox potential

Using microflotation method, this study explored the collectorless flotation of Chalcocite and its dependence on the redox potential of pulp . Electrochemical studies were performed by cyclic voltammetry  in specific potential ranges and at different pH values. The results show that significant floatability of Chalcocite occurs in the specific reducing conditions. By increasing potentials, on t...

Expression system of CotA-laccase for directed evolution and high-throughput screenings for the oxidation of high-redox potential dyes.

Laccases are useful biocatalysts for many diverse biotechnological applications. In this study we have established efficient and reliable expression systems and high-throughput screenings for the recombinant CotA-laccase from Bacillus subtilis. The expression levels of cotA-laccase were compared in five different Escherichia coli host strains growing in 96-well microtiter plates under different...

Lignin Model Compound Mediators in Oxidation of a Nonphenolic Comparison of Fungal Laccases and Redox